3-D Life PVA-PEG Hydrogel FG

Catalog Number: FG80-1

Unit Size: 1 Kit

Shipping Temperature: Dry Ice

87,00 €

Add to cart
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  • Description
  • Specifications

Details
Supporting Information

Description The 3-D Life PVA-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized polyvinyl alcohol (Mal-PVA) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on Mal-PVA, which results in the formation of the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-PVA to provide a cell-adhesive matrix.

For more information and instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.
Catalog Number FG80-1
Application 3-D cell cultures, hydrogel barriers.
Quantity Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.
Components
Material Quantity Concentration
Mal-PVA170 µl30 mmol/L maleimide groups
PEG-Link, lyophilized200 µl*20 mmol/L* thiol groups
10x CB (pH 5.5)200 µln.a
Water2 x 1500 µln.a
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

GP-1: Fast-gelling Hydrogels

Applications

Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels
Application Note 4: Hydrogel stiffness
Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels

User Guide

3-D Life Hydrogel User Guide

Data Sheet

Detailed Product Information

Dry ice

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Details
Supporting Information

Description The 3-D Life PVA-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized polyvinyl alcohol (Mal-PVA) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on Mal-PVA, which results in the formation of the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-PVA to provide a cell-adhesive matrix.

For more information and instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.
Catalog Number FG80-1
Application 3-D cell cultures, hydrogel barriers.
Quantity Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.
Components
Material Quantity Concentration
Mal-PVA170 µl30 mmol/L maleimide groups
PEG-Link, lyophilized200 µl*20 mmol/L* thiol groups
10x CB (pH 5.5)200 µln.a
Water2 x 1500 µln.a
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

GP-1: Fast-gelling Hydrogels

Applications

Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels
Application Note 4: Hydrogel stiffness
Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels

User Guide

3-D Life Hydrogel User Guide

Data Sheet

Detailed Product Information

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Details
Supporting Information

Description The 3-D Life PVA-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized polyvinyl alcohol (Mal-PVA) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on Mal-PVA, which results in the formation of the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-PVA to provide a cell-adhesive matrix.

For more information and instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.
Catalog Number FG80-1
Application 3-D cell cultures, hydrogel barriers.
Quantity Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.
Components
Material Quantity Concentration
Mal-PVA170 µl30 mmol/L maleimide groups
PEG-Link, lyophilized200 µl*20 mmol/L* thiol groups
10x CB (pH 5.5)200 µln.a
Water2 x 1500 µln.a
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

GP-1: Fast-gelling Hydrogels

Applications

Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels
Application Note 4: Hydrogel stiffness
Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels

User Guide

3-D Life Hydrogel User Guide

Data Sheet

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Details
Supporting Information

Description The 3-D Life PVA-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized polyvinyl alcohol (Mal-PVA) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on Mal-PVA, which results in the formation of the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-PVA to provide a cell-adhesive matrix.

For more information and instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.
Catalog Number FG80-1
Application 3-D cell cultures, hydrogel barriers.
Quantity Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.
Components
Material Quantity Concentration
Mal-PVA170 µl30 mmol/L maleimide groups
PEG-Link, lyophilized200 µl*20 mmol/L* thiol groups
10x CB (pH 5.5)200 µln.a
Water2 x 1500 µln.a
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

GP-1: Fast-gelling Hydrogels

Applications

Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels
Application Note 4: Hydrogel stiffness
Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels

User Guide

3-D Life Hydrogel User Guide

Data Sheet

Detailed Product Information

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Details
Supporting Information

Description The 3-D Life PVA-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized polyvinyl alcohol (Mal-PVA) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on Mal-PVA, which results in the formation of the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-PVA to provide a cell-adhesive matrix.

For more information and instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.
Catalog Number FG80-1
Application 3-D cell cultures, hydrogel barriers.
Quantity Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.
Components
Material Quantity Concentration
Mal-PVA170 µl30 mmol/L maleimide groups
PEG-Link, lyophilized200 µl*20 mmol/L* thiol groups
10x CB (pH 5.5)200 µln.a
Water2 x 1500 µln.a
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

GP-1: Fast-gelling Hydrogels

Applications

Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels
Application Note 4: Hydrogel stiffness
Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels

User Guide

3-D Life Hydrogel User Guide

Data Sheet

Detailed Product Information

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Details
Supporting Information

Description The 3-D Life PVA-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized polyvinyl alcohol (Mal-PVA) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on Mal-PVA, which results in the formation of the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-PVA to provide a cell-adhesive matrix.

For more information and instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.
Catalog Number FG80-1
Application 3-D cell cultures, hydrogel barriers.
Quantity Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.
Components
Material Quantity Concentration
Mal-PVA170 µl30 mmol/L maleimide groups
PEG-Link, lyophilized200 µl*20 mmol/L* thiol groups
10x CB (pH 5.5)200 µln.a
Water2 x 1500 µln.a
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

GP-1: Fast-gelling Hydrogels

Applications

Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels
Application Note 4: Hydrogel stiffness
Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels

User Guide

3-D Life Hydrogel User Guide

Data Sheet

Detailed Product Information

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Details
Supporting Information

Description The 3-D Life PVA-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized polyvinyl alcohol (Mal-PVA) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on Mal-PVA, which results in the formation of the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-PVA to provide a cell-adhesive matrix.

For more information and instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.
Catalog Number FG80-1
Application 3-D cell cultures, hydrogel barriers.
Quantity Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.
Components
Material Quantity Concentration
Mal-PVA170 µl30 mmol/L maleimide groups
PEG-Link, lyophilized200 µl*20 mmol/L* thiol groups
10x CB (pH 5.5)200 µln.a
Water2 x 1500 µln.a
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

GP-1: Fast-gelling Hydrogels

Applications

Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels
Application Note 4: Hydrogel stiffness
Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels

User Guide

3-D Life Hydrogel User Guide

Data Sheet

Detailed Product Information

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