ToLuminate Photogels

ToLuminate Photogels are photo-crosslinkable hydrogels (Photogels). Kits for forming Photogels contain all necessary components, including norbornene-modified dextran that reacts with 3-D Life crosslinkers on the basis of a thiol-ene reaction. After mixing of the hydrogel components gelation is initiated at any time with light at wavelengths of 365-405 nm. The hydrogels can be adjusted to various cell culture conditions by attachment of cell adhesion peptides or inclusion of matrix metalloproteinase-cleavable motifs.

ToLuminate photogels crosslinked with PEG-Link or CD-Link can be dissolved by 3-D Life Dextranase for a protease-free cell recovery.

Cell adhesion peptides, Dextranase for gel dissolution and other gel modifying reagents are available as separate products (see products Adhesion Peptides and Accessories).

Hydrogel compositions with specific properties can be identified wth the hydrogel selection tool.

CD or PEG: Choose between a cell-degradable (CD) crosslinker and a non-degradable (PEG) crosslinker.

HA or CD-HA: Choose a hyaluronic acid-based crosslinker, either hyaluronic acid only (HA) or one with a matrix metalloproteinase-cleavable motif (CD-HA).