<span id="hikashop_product_name_main" class="hikashop_product_name_main" itemprop="name"> 3-D Life PVA-PEG Hydrogel SG

User Guide

Data Sheet

FG80-1
1 Kit

Shipping Condition: Dry ice

87,00 €

3-D Life SG-PVA

Details

Supporting Information

Description

The 3-D Life PVA-PEG Hydrogel SG Kit provides reagents for setting up slow gelling, cell-compatible hydrogels. Its major components are the polymeric SG-PVA and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with thiol-reactive groups on SG-PVA, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation.Prior to the crosslinking step, peptides (e.g. 3-D Life RGD Peptide, Cat.No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups of SG-PVA to provide a cell-adhesive matrix. For more information and instructions, please consult the General Protocol GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

G82-1

Application

Quantity

Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.

Components

Material	Quantity	Concentration
SG-PVA	170 µl	30 mmol/L thioreactive groups
PEG-Link, lyophilized	200 µl*	20 mmol/L* thiol groups
10x CB (pH 7.2)	200 µl	n.a.
Water	2 x 1500 µl	n.a
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

User Guide

Data Sheet

M81-3
3x 170 µl

Shipping Condition: Ambient

158,00 €

3-D Life PEG-Link

Details

Supporting Information

Description

The 3-D Life PVA-PEG Hydrogel SG Kit provides reagents for setting up slow gelling, cell-compatible hydrogels. Its major components are the polymeric SG-PVA and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with thiol-reactive groups on SG-PVA, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation.Prior to the crosslinking step, peptides (e.g. 3-D Life RGD Peptide, Cat.No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups of SG-PVA to provide a cell-adhesive matrix. For more information and instructions, please consult the General Protocol GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

G82-1

Application

Quantity

Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.

Components

Material	Quantity	Concentration
SG-PVA	170 µl	30 mmol/L thioreactive groups
PEG-Link, lyophilized	200 µl*	20 mmol/L* thiol groups
10x CB (pH 7.2)	200 µl	n.a.
Water	2 x 1500 µl	n.a
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

User Guide

Data Sheet

L50-1
200 µl

Shipping Condition: Ambient

40,00 €

3-D Life PEG-Link

Details

Supporting Information

Description

The 3-D Life PVA-PEG Hydrogel SG Kit provides reagents for setting up slow gelling, cell-compatible hydrogels. Its major components are the polymeric SG-PVA and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with thiol-reactive groups on SG-PVA, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation.Prior to the crosslinking step, peptides (e.g. 3-D Life RGD Peptide, Cat.No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups of SG-PVA to provide a cell-adhesive matrix. For more information and instructions, please consult the General Protocol GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

G82-1

Application

Quantity

Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.

Components

Material	Quantity	Concentration
SG-PVA	170 µl	30 mmol/L thioreactive groups
PEG-Link, lyophilized	200 µl*	20 mmol/L* thiol groups
10x CB (pH 7.2)	200 µl	n.a.
Water	2 x 1500 µl	n.a
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

User Guide

Data Sheet

L50-3
3x 200 µl

Shipping Condition: Ambient

87,00 €

3-D Life 10x CB-Buffer (pH 7.2)

Details

Supporting Information

Description

The 3-D Life PVA-PEG Hydrogel SG Kit provides reagents for setting up slow gelling, cell-compatible hydrogels. Its major components are the polymeric SG-PVA and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with thiol-reactive groups on SG-PVA, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation.Prior to the crosslinking step, peptides (e.g. 3-D Life RGD Peptide, Cat.No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups of SG-PVA to provide a cell-adhesive matrix. For more information and instructions, please consult the General Protocol GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

G82-1

Application

Quantity

Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.

Components

Material	Quantity	Concentration
SG-PVA	170 µl	30 mmol/L thioreactive groups
PEG-Link, lyophilized	200 µl*	20 mmol/L* thiol groups
10x CB (pH 7.2)	200 µl	n.a.
Water	2 x 1500 µl	n.a
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

User Guide

Data Sheet