ToLuminate CD Photogel

Catalog Number: PG91-1

Unit Size: 1 Kit

Shipping Temperature: Ambient

420,00 €

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  • Description
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Details
Supporting Information

Description The ToLuminate CD Photogel kit provides reagents for setting up cell-compatible hydrogels formed by illumination with blue light (365-405 nm) on the basis of the thiol-ene chemistry. Its major components are norbornene-modified dextran (N-Dextran) and the thiol-modified crosslinker CD-Link. When the two reagents are combined and illuminated in the presence of LAP (Lithium phenyl-2,4,6-trimethylbenzoylphosphinate) thiol groups on CD-Link are activated and form stable thioether bonds with norbornene groups on N-Dextran. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. There is ample time to place the pre-gel solution in any culture dish or container, since gelation starts only after illumination.

CD-Link contains a matrix metalloproteinase (MMP)-cleavable peptide sequence (Pro-Leu-Gly-Leu-Trp-Ala), which is cleaved by a broad range of MMPs including MMP1,MMP2, MMP3, MMP7, MMP9 and MMP13 [1, 2, 3]. It allows cells to locally degrade the polymer network, if they produce the indicated MMPs.

Cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the norbornene groups on N-Dextran with the same chemical reaction as CD-Link to provide a cell-adhesive matrix.

3-D Life AgaFloat (Cat. No. A10-3) can be added to the pre-gel solution to avoid sedimentation of cells before illumination.

Dextran hydrogels crosslinked with CD-Link can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

For more information and instructions, please consult the General Protocol GP-5 "Preparation of ToLuminate Photogels".
Catalog Number PG91-1
Application 3-D cell culture, bioprinting.
Quantity Up to 4 mL hydrogel.
Components
Material Quantity Concentration
N-Dextran600 µl30 mmol/L
CD-Link, lyophilized3 x 200 µl*20 mmol/L* thiol groups
10x CB (pH 7.2) Phenol Red-free600 µln/a
LAP, 10 mM600 µl10 mmol/L
Water2 x 1500 µln.a
*Volume/concentration after reconstitution of lyophilisate.
Reference[1] Knight, C. G. et al. FEBS 296:263-66 (1992) [2] Patterson, J. and Hubbell, J. A. Biomaterials 31, 7836-7845 (2010) [3] Deng, S. et al. J. Biol. Chem. 275, No. 40, 31422–31427 (2000)

General Protocols

GP-5: ToLuminate Photogels

Technical Protocols

TP1: Degradation of dextran-based hydrogels with dextranase.

Data Sheet

Detailed Product Information

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