ToLuminate N-Dextran

Catalog Number: M93-2

Unit Size: 2x 600 µL

Shipping Temperature: Ambient

230,00 €

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  • Description
  • Specifications

Details
Supporting Information

Description ToLuminate N-Dextran is a component of the ToLuminate Hydrogel system. It contains norbornene-functionalized dextran (N-Dextran) that can be used together with 3-D Life crosslinkers PEG-Link (Cat. No. L50-1 or L50-3), CD-Link (Cat. No. L60-1 or L60-3), HyLink (Cat. No. L70-1 or L70-3) or CD-HyLink (L80-1 or L80-3) to set up hydrogels formed by illumination with blue light (365-405 nm) on the basis of the thiol-ene chemistry. In addition, the set contains the photoinitiator LAP (Lithium phenyl-2,4,6-trimethyl-benzoylphosphinate) required for photocrosslinking and a phenol red-free 10x buffer solution compatible with light-dependent crosslinking.

When N-Dextran is combined with crosslinker and illuminated in the presence of LAP, thiol groups on the crosslinker are activated and form stable thioether bonds with norbornene groups on N-Dextran.

When crosslinked with PEG-Link or CD-Link, dextran-based ToLuminate Hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

The 10x CB (pH 7.2) Phenol Red-free is a buffer solution to control pH and osmotic conditions during gel formation in the presence of cells.

For more information and instructions, please consult the General Protocol GP-5 "Preparation of ToLuminate Photogels".
Catalog Number M93-2
Application 3D cell cultures, bioprinting
Quantity Without peptide modification:
  • Up to 18 mL hydrogel at a crosslinking strength of 2 mmol/L (used for crosslinking with HyLink or CD-HyLink).
  • Up to 12 mL hydrogel at a crosslinking strength of 3 mmol/L (used for crosslinking with PEG-Link or CD-Link).
The hydrogel volume will be reduced, if peptide modifications are added.
Components
Material Quantity Concentration
N-Dextran2 x 600 µl30 mmol/L
10x CB (pH 7.2) Phenol Red-free2 x 600 µln/a
LAP, 10 mM2 x 600 µl10 mmol/L
Water3 x 1500 µln.a

General Protocols

GP-5: ToLuminate Photogels

Technical Protocols

TP1: Degradation of dextran-based hydrogels with dextranase.

Data Sheet

Detailed Product Information

Ambient

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pg90-1_781951107
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ToLuminate PEG Photogel
2025-03-28 10:54

Details
Supporting Information

Description ToLuminate N-Dextran is a component of the ToLuminate Hydrogel system. It contains norbornene-functionalized dextran (N-Dextran) that can be used together with 3-D Life crosslinkers PEG-Link (Cat. No. L50-1 or L50-3), CD-Link (Cat. No. L60-1 or L60-3), HyLink (Cat. No. L70-1 or L70-3) or CD-HyLink (L80-1 or L80-3) to set up hydrogels formed by illumination with blue light (365-405 nm) on the basis of the thiol-ene chemistry. In addition, the set contains the photoinitiator LAP (Lithium phenyl-2,4,6-trimethyl-benzoylphosphinate) required for photocrosslinking and a phenol red-free 10x buffer solution compatible with light-dependent crosslinking.

When N-Dextran is combined with crosslinker and illuminated in the presence of LAP, thiol groups on the crosslinker are activated and form stable thioether bonds with norbornene groups on N-Dextran.

When crosslinked with PEG-Link or CD-Link, dextran-based ToLuminate Hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

The 10x CB (pH 7.2) Phenol Red-free is a buffer solution to control pH and osmotic conditions during gel formation in the presence of cells.

For more information and instructions, please consult the General Protocol GP-5 "Preparation of ToLuminate Photogels".
Catalog Number M93-2
Application 3D cell cultures, bioprinting
Quantity Without peptide modification:
  • Up to 18 mL hydrogel at a crosslinking strength of 2 mmol/L (used for crosslinking with HyLink or CD-HyLink).
  • Up to 12 mL hydrogel at a crosslinking strength of 3 mmol/L (used for crosslinking with PEG-Link or CD-Link).
The hydrogel volume will be reduced, if peptide modifications are added.
Components
Material Quantity Concentration
N-Dextran2 x 600 µl30 mmol/L
10x CB (pH 7.2) Phenol Red-free2 x 600 µln/a
LAP, 10 mM2 x 600 µl10 mmol/L
Water3 x 1500 µln.a

General Protocols

GP-5: ToLuminate Photogels

Technical Protocols

TP1: Degradation of dextran-based hydrogels with dextranase.

Data Sheet

Detailed Product Information

PG90-1
1 Kit
Ambient
200,00 €
Add to cart

pg91-1_818235238
PC-CD
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ToLuminate CD Photogel
2025-06-27 17:44

Details
Supporting Information

Description ToLuminate N-Dextran is a component of the ToLuminate Hydrogel system. It contains norbornene-functionalized dextran (N-Dextran) that can be used together with 3-D Life crosslinkers PEG-Link (Cat. No. L50-1 or L50-3), CD-Link (Cat. No. L60-1 or L60-3), HyLink (Cat. No. L70-1 or L70-3) or CD-HyLink (L80-1 or L80-3) to set up hydrogels formed by illumination with blue light (365-405 nm) on the basis of the thiol-ene chemistry. In addition, the set contains the photoinitiator LAP (Lithium phenyl-2,4,6-trimethyl-benzoylphosphinate) required for photocrosslinking and a phenol red-free 10x buffer solution compatible with light-dependent crosslinking.

When N-Dextran is combined with crosslinker and illuminated in the presence of LAP, thiol groups on the crosslinker are activated and form stable thioether bonds with norbornene groups on N-Dextran.

When crosslinked with PEG-Link or CD-Link, dextran-based ToLuminate Hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

The 10x CB (pH 7.2) Phenol Red-free is a buffer solution to control pH and osmotic conditions during gel formation in the presence of cells.

For more information and instructions, please consult the General Protocol GP-5 "Preparation of ToLuminate Photogels".
Catalog Number M93-2
Application 3D cell cultures, bioprinting
Quantity Without peptide modification:
  • Up to 18 mL hydrogel at a crosslinking strength of 2 mmol/L (used for crosslinking with HyLink or CD-HyLink).
  • Up to 12 mL hydrogel at a crosslinking strength of 3 mmol/L (used for crosslinking with PEG-Link or CD-Link).
The hydrogel volume will be reduced, if peptide modifications are added.
Components
Material Quantity Concentration
N-Dextran2 x 600 µl30 mmol/L
10x CB (pH 7.2) Phenol Red-free2 x 600 µln/a
LAP, 10 mM2 x 600 µl10 mmol/L
Water3 x 1500 µln.a

General Protocols

GP-5: ToLuminate Photogels

Technical Protocols

TP1: Degradation of dextran-based hydrogels with dextranase.

Data Sheet

Detailed Product Information

PG91-1
1 Kit
Ambient
420,00 €
Add to cart

pg95-1
PC-HA
New3
ToLuminate HA Photogel
2025-11-18 15:20

Details
Supporting Information

Description ToLuminate N-Dextran is a component of the ToLuminate Hydrogel system. It contains norbornene-functionalized dextran (N-Dextran) that can be used together with 3-D Life crosslinkers PEG-Link (Cat. No. L50-1 or L50-3), CD-Link (Cat. No. L60-1 or L60-3), HyLink (Cat. No. L70-1 or L70-3) or CD-HyLink (L80-1 or L80-3) to set up hydrogels formed by illumination with blue light (365-405 nm) on the basis of the thiol-ene chemistry. In addition, the set contains the photoinitiator LAP (Lithium phenyl-2,4,6-trimethyl-benzoylphosphinate) required for photocrosslinking and a phenol red-free 10x buffer solution compatible with light-dependent crosslinking.

When N-Dextran is combined with crosslinker and illuminated in the presence of LAP, thiol groups on the crosslinker are activated and form stable thioether bonds with norbornene groups on N-Dextran.

When crosslinked with PEG-Link or CD-Link, dextran-based ToLuminate Hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

The 10x CB (pH 7.2) Phenol Red-free is a buffer solution to control pH and osmotic conditions during gel formation in the presence of cells.

For more information and instructions, please consult the General Protocol GP-5 "Preparation of ToLuminate Photogels".
Catalog Number M93-2
Application 3D cell cultures, bioprinting
Quantity Without peptide modification:
  • Up to 18 mL hydrogel at a crosslinking strength of 2 mmol/L (used for crosslinking with HyLink or CD-HyLink).
  • Up to 12 mL hydrogel at a crosslinking strength of 3 mmol/L (used for crosslinking with PEG-Link or CD-Link).
The hydrogel volume will be reduced, if peptide modifications are added.
Components
Material Quantity Concentration
N-Dextran2 x 600 µl30 mmol/L
10x CB (pH 7.2) Phenol Red-free2 x 600 µln/a
LAP, 10 mM2 x 600 µl10 mmol/L
Water3 x 1500 µln.a

General Protocols

GP-5: ToLuminate Photogels

Technical Protocols

TP1: Degradation of dextran-based hydrogels with dextranase.

Data Sheet

Detailed Product Information

PG95-1
1 Kit
Ambient
330,00 €
Add to cart

pg96-1
PC-CD-HA
New3
ToLuminate CD-HA Photogel
2025-11-18 15:21

Details
Supporting Information

Description ToLuminate N-Dextran is a component of the ToLuminate Hydrogel system. It contains norbornene-functionalized dextran (N-Dextran) that can be used together with 3-D Life crosslinkers PEG-Link (Cat. No. L50-1 or L50-3), CD-Link (Cat. No. L60-1 or L60-3), HyLink (Cat. No. L70-1 or L70-3) or CD-HyLink (L80-1 or L80-3) to set up hydrogels formed by illumination with blue light (365-405 nm) on the basis of the thiol-ene chemistry. In addition, the set contains the photoinitiator LAP (Lithium phenyl-2,4,6-trimethyl-benzoylphosphinate) required for photocrosslinking and a phenol red-free 10x buffer solution compatible with light-dependent crosslinking.

When N-Dextran is combined with crosslinker and illuminated in the presence of LAP, thiol groups on the crosslinker are activated and form stable thioether bonds with norbornene groups on N-Dextran.

When crosslinked with PEG-Link or CD-Link, dextran-based ToLuminate Hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

The 10x CB (pH 7.2) Phenol Red-free is a buffer solution to control pH and osmotic conditions during gel formation in the presence of cells.

For more information and instructions, please consult the General Protocol GP-5 "Preparation of ToLuminate Photogels".
Catalog Number M93-2
Application 3D cell cultures, bioprinting
Quantity Without peptide modification:
  • Up to 18 mL hydrogel at a crosslinking strength of 2 mmol/L (used for crosslinking with HyLink or CD-HyLink).
  • Up to 12 mL hydrogel at a crosslinking strength of 3 mmol/L (used for crosslinking with PEG-Link or CD-Link).
The hydrogel volume will be reduced, if peptide modifications are added.
Components
Material Quantity Concentration
N-Dextran2 x 600 µl30 mmol/L
10x CB (pH 7.2) Phenol Red-free2 x 600 µln/a
LAP, 10 mM2 x 600 µl10 mmol/L
Water3 x 1500 µln.a

General Protocols

GP-5: ToLuminate Photogels

Technical Protocols

TP1: Degradation of dextran-based hydrogels with dextranase.

Data Sheet

Detailed Product Information

PG96-1
1 Kit
Ambient
490,00 €
Add to cart
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