Dextran-PEG Hydrogel FG

Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels

Applications

Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels

User Guide

Data Sheet

ced2

{/tabs}

FG80-1
1 Kit

Shipping Condition: Dry ice

87,00 €

3-D Life FG-Dextran

{tab title="Details"}

Description

The 3-D Life Dextran-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized dextran (Mal-Dextran) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on the Mal-Dextran, which results in the formation of the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-Dextran to provide a cell-adhesive matrix.

Dextran hydrogels crosslinked with PEG-Link can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

FG90-1

Application

3-D cell cultures requiring no cell spreading and migration; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.

Quantity

Allows formation of up to 2 ml 3-D Life Hydrogel depending on the grade of stiffness of the gel.

Components

Material	Quantity	Concentration
Mal-Dextran, lyophilized	170 µl*	30 mmol/L* maleimide groups
PEG-Link, lyophilized	200 µl*	20 mmol/L* thiol groups
10x CB (pH 5.5)	200 µl	n.a
Water	2 x 1500 µl	n.a
*Volume/concentration after reconstitution of lyophilisate.

{tab title="Supporting Information"}

General Protocols

Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels

Applications

Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels

User Guide

Data Sheet

ced2

{/tabs}

M92-3
3x 170 µl

Shipping Condition: Ambient

168,00 €

3-D Life PEG-Link

{tab title="Details"}

Description

The 3-D Life Dextran-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized dextran (Mal-Dextran) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on the Mal-Dextran, which results in the formation of the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-Dextran to provide a cell-adhesive matrix.

Dextran hydrogels crosslinked with PEG-Link can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

FG90-1

Application

3-D cell cultures requiring no cell spreading and migration; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.

Quantity

Allows formation of up to 2 ml 3-D Life Hydrogel depending on the grade of stiffness of the gel.

Components

Material	Quantity	Concentration
Mal-Dextran, lyophilized	170 µl*	30 mmol/L* maleimide groups
PEG-Link, lyophilized	200 µl*	20 mmol/L* thiol groups
10x CB (pH 5.5)	200 µl	n.a
Water	2 x 1500 µl	n.a
*Volume/concentration after reconstitution of lyophilisate.

{tab title="Supporting Information"}

General Protocols

Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels

Applications

Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels

User Guide

Data Sheet

ced2

{/tabs}

L50-1
200 µl

Shipping Condition: Ambient

40,00 €

3-D Life PEG-Link

{tab title="Details"}

Description

The 3-D Life Dextran-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized dextran (Mal-Dextran) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on the Mal-Dextran, which results in the formation of the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-Dextran to provide a cell-adhesive matrix.

Dextran hydrogels crosslinked with PEG-Link can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

FG90-1

Application

3-D cell cultures requiring no cell spreading and migration; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.

Quantity

Allows formation of up to 2 ml 3-D Life Hydrogel depending on the grade of stiffness of the gel.

Components

Material	Quantity	Concentration
Mal-Dextran, lyophilized	170 µl*	30 mmol/L* maleimide groups
PEG-Link, lyophilized	200 µl*	20 mmol/L* thiol groups
10x CB (pH 5.5)	200 µl	n.a
Water	2 x 1500 µl	n.a
*Volume/concentration after reconstitution of lyophilisate.

{tab title="Supporting Information"}

General Protocols

Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels

Applications

Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels

User Guide

Data Sheet

ced2

{/tabs}

L50-3
3x 200 µl

Shipping Condition: Ambient

87,00 €

3-D Life 10x CB-Buffer (pH 5.5)

{tab title="Details"}

Description

The 3-D Life Dextran-PEG Hydrogel FG Kit provides reagents for setting up fast gelling, cell-compatible hydrogels. Its major components are maleimide-functionalized dextran (Mal-Dextran) and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with maleimide groups on the Mal-Dextran, which results in the formation of the gel.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the maleimide groups of Mal-Dextran to provide a cell-adhesive matrix.

Dextran hydrogels crosslinked with PEG-Link can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

FG90-1

Application

3-D cell cultures requiring no cell spreading and migration; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.

Quantity

Allows formation of up to 2 ml 3-D Life Hydrogel depending on the grade of stiffness of the gel.

Components

Material	Quantity	Concentration
Mal-Dextran, lyophilized	170 µl*	30 mmol/L* maleimide groups
PEG-Link, lyophilized	200 µl*	20 mmol/L* thiol groups
10x CB (pH 5.5)	200 µl	n.a
Water	2 x 1500 µl	n.a
*Volume/concentration after reconstitution of lyophilisate.

{tab title="Supporting Information"}

General Protocols

Application Note: Epithelial Cyst Formation in 3-D Life Hydrogels

Applications

Application Protocol: MDCK Cyst Formation in 3-D Life Hydrogels

User Guide

Data Sheet