ToLuminate HA Photogel

Catalog Number: PG95-1

Unit Size: 1 Kit

Shipping Temperature: Ambient

330,00 €

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Supporting Information

Description The ToLuminate HA Photogel kit provides reagents for setting up cell-compatible hydrogels formed by illumination with blue light (365-405 nm) on the basis of the thiol-ene chemistry. Its major components are norbornene-modified dextran (N-Dextran) and the thiol-modified hyaluronic acid crosslinker HyLink. When the two reagents are combined and illuminated in the presence of LAP (Lithium phenyl-2,4,6-trimethylbenzoylphosphinate) thiol groups on HyLink are activated and form stable thioether bonds with norbornene groups on N-Dextran. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. There is ample time to place the pre-gel solution in any culture dish or container, since gelation starts only after illumination. The hydrogel allows cell spreading and migration if cell adhesion peptides are present in the gel.

Cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups on N-Dextran to provide a cell-adhesive matrix.

3-D Life AgaFloat (Cat. No. A10-3) can be added to the pre-gel solution to avoid sedimentation of cells before illumination.

Note: Dextran hydrogels crosslinked with HyLink cannot be dissolved by the addition of dextranase.

For more information and instructions, please consult the General Protocol GP-5 "Preparation of ToLuminate Photogels".
Catalog Number PG95-1
Application 3-D cell culture, bioprinting.
Quantity Up to 4 mL hydrogel.
Components
Material Quantity Concentration
N-Dextran600 µl30 mmol/L
HyLink, lyophilized4 x 200 µl*10 mmol/L* thiol groups
10x CB (pH 7.2) Phenol Red-free600 µln/a
LAP, 10 mM600 µl10 mmol/L
Water2 x 1500 µln.a
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

GP-5: ToLuminate Photogels

Data Sheet

Detailed Product Information

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