ToLuminate HA Photogel

Details

Supporting Information

Description

The ToLuminate HA Photogel kit provides reagents for setting up cell-compatible hydrogels formed by illumination with blue light (365-405 nm) on the basis of the thiol-ene chemistry. Its major components are norbornene-modified dextran (N-Dextran) and the thiol-modified hyaluronic acid crosslinker HyLink. When the two reagents are combined and illuminated in the presence of LAP (Lithium phenyl-2,4,6-trimethylbenzoylphosphinate) thiol groups on HyLink are activated and form stable thioether bonds with norbornene groups on N-Dextran. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. There is ample time to place the pre-gel solution in any culture dish or container, since gelation starts only after illumination. The hydrogel allows cell spreading and migration if cell adhesion peptides are present in the gel.

Cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups on N-Dextran to provide a cell-adhesive matrix.

3-D Life AgaFloat (Cat. No. A10-3) can be added to the pre-gel solution to avoid sedimentation of cells before illumination.

Note: Dextran hydrogels crosslinked with HyLink cannot be dissolved by the addition of dextranase.

For more information and instructions, please consult the General Protocol GP-5 "Preparation of ToLuminate Photogels".

Catalog Number

PG95-1

Application

3-D cell culture, bioprinting.

Quantity

Up to 4 mL hydrogel.

Components

Material	Quantity	Concentration
N-Dextran	600 µl	30 mmol/L
HyLink, lyophilized	4 x 200 µl*	10 mmol/L* thiol groups
10x CB (pH 7.2) Phenol Red-free	600 µl	n/a
LAP, 10 mM	600 µl	10 mmol/L
Water	2 x 1500 µl	n.a
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

GP-5: ToLuminate Photogels

Data Sheet

Detailed Product Information

PG90-1
1 Kit

Shipping Condition: Ambient

200,00 €

ToLuminate CD Photogel

Details

Supporting Information

Description

The ToLuminate HA Photogel kit provides reagents for setting up cell-compatible hydrogels formed by illumination with blue light (365-405 nm) on the basis of the thiol-ene chemistry. Its major components are norbornene-modified dextran (N-Dextran) and the thiol-modified hyaluronic acid crosslinker HyLink. When the two reagents are combined and illuminated in the presence of LAP (Lithium phenyl-2,4,6-trimethylbenzoylphosphinate) thiol groups on HyLink are activated and form stable thioether bonds with norbornene groups on N-Dextran. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. There is ample time to place the pre-gel solution in any culture dish or container, since gelation starts only after illumination. The hydrogel allows cell spreading and migration if cell adhesion peptides are present in the gel.

Cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups on N-Dextran to provide a cell-adhesive matrix.

3-D Life AgaFloat (Cat. No. A10-3) can be added to the pre-gel solution to avoid sedimentation of cells before illumination.

Note: Dextran hydrogels crosslinked with HyLink cannot be dissolved by the addition of dextranase.

For more information and instructions, please consult the General Protocol GP-5 "Preparation of ToLuminate Photogels".

Catalog Number

PG95-1

Application

3-D cell culture, bioprinting.

Quantity

Up to 4 mL hydrogel.

Components

Material	Quantity	Concentration
N-Dextran	600 µl	30 mmol/L
HyLink, lyophilized	4 x 200 µl*	10 mmol/L* thiol groups
10x CB (pH 7.2) Phenol Red-free	600 µl	n/a
LAP, 10 mM	600 µl	10 mmol/L
Water	2 x 1500 µl	n.a
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

GP-5: ToLuminate Photogels

Data Sheet

Detailed Product Information

PG91-1
1 Kit

Shipping Condition: Ambient

420,00 €

ToLuminate CD-HA Photogel

Details

Supporting Information

Description

The ToLuminate HA Photogel kit provides reagents for setting up cell-compatible hydrogels formed by illumination with blue light (365-405 nm) on the basis of the thiol-ene chemistry. Its major components are norbornene-modified dextran (N-Dextran) and the thiol-modified hyaluronic acid crosslinker HyLink. When the two reagents are combined and illuminated in the presence of LAP (Lithium phenyl-2,4,6-trimethylbenzoylphosphinate) thiol groups on HyLink are activated and form stable thioether bonds with norbornene groups on N-Dextran. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. There is ample time to place the pre-gel solution in any culture dish or container, since gelation starts only after illumination. The hydrogel allows cell spreading and migration if cell adhesion peptides are present in the gel.

Cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups on N-Dextran to provide a cell-adhesive matrix.

3-D Life AgaFloat (Cat. No. A10-3) can be added to the pre-gel solution to avoid sedimentation of cells before illumination.

Note: Dextran hydrogels crosslinked with HyLink cannot be dissolved by the addition of dextranase.

For more information and instructions, please consult the General Protocol GP-5 "Preparation of ToLuminate Photogels".

Catalog Number

PG95-1

Application

3-D cell culture, bioprinting.

Quantity

Up to 4 mL hydrogel.

Components

Material	Quantity	Concentration
N-Dextran	600 µl	30 mmol/L
HyLink, lyophilized	4 x 200 µl*	10 mmol/L* thiol groups
10x CB (pH 7.2) Phenol Red-free	600 µl	n/a
LAP, 10 mM	600 µl	10 mmol/L
Water	2 x 1500 µl	n.a
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

GP-5: ToLuminate Photogels

Data Sheet

Detailed Product Information

PG96-1
1 Kit

Shipping Condition: Ambient

490,00 €

ToLuminate N-Dextran

Details

Supporting Information

Description

The ToLuminate HA Photogel kit provides reagents for setting up cell-compatible hydrogels formed by illumination with blue light (365-405 nm) on the basis of the thiol-ene chemistry. Its major components are norbornene-modified dextran (N-Dextran) and the thiol-modified hyaluronic acid crosslinker HyLink. When the two reagents are combined and illuminated in the presence of LAP (Lithium phenyl-2,4,6-trimethylbenzoylphosphinate) thiol groups on HyLink are activated and form stable thioether bonds with norbornene groups on N-Dextran. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. There is ample time to place the pre-gel solution in any culture dish or container, since gelation starts only after illumination. The hydrogel allows cell spreading and migration if cell adhesion peptides are present in the gel.

Cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups on N-Dextran to provide a cell-adhesive matrix.

3-D Life AgaFloat (Cat. No. A10-3) can be added to the pre-gel solution to avoid sedimentation of cells before illumination.

Note: Dextran hydrogels crosslinked with HyLink cannot be dissolved by the addition of dextranase.

For more information and instructions, please consult the General Protocol GP-5 "Preparation of ToLuminate Photogels".

Catalog Number

PG95-1

Application

3-D cell culture, bioprinting.

Quantity

Up to 4 mL hydrogel.

Components

Material	Quantity	Concentration
N-Dextran	600 µl	30 mmol/L
HyLink, lyophilized	4 x 200 µl*	10 mmol/L* thiol groups
10x CB (pH 7.2) Phenol Red-free	600 µl	n/a
LAP, 10 mM	600 µl	10 mmol/L
Water	2 x 1500 µl	n.a
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

GP-5: ToLuminate Photogels

Data Sheet

Detailed Product Information

M93-2
2x 600 µL

Shipping Condition: Ambient

230,00 €

3-D Life HyLink

Details

Supporting Information

Description

The ToLuminate HA Photogel kit provides reagents for setting up cell-compatible hydrogels formed by illumination with blue light (365-405 nm) on the basis of the thiol-ene chemistry. Its major components are norbornene-modified dextran (N-Dextran) and the thiol-modified hyaluronic acid crosslinker HyLink. When the two reagents are combined and illuminated in the presence of LAP (Lithium phenyl-2,4,6-trimethylbenzoylphosphinate) thiol groups on HyLink are activated and form stable thioether bonds with norbornene groups on N-Dextran. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. There is ample time to place the pre-gel solution in any culture dish or container, since gelation starts only after illumination. The hydrogel allows cell spreading and migration if cell adhesion peptides are present in the gel.

Cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups on N-Dextran to provide a cell-adhesive matrix.

3-D Life AgaFloat (Cat. No. A10-3) can be added to the pre-gel solution to avoid sedimentation of cells before illumination.

Note: Dextran hydrogels crosslinked with HyLink cannot be dissolved by the addition of dextranase.

For more information and instructions, please consult the General Protocol GP-5 "Preparation of ToLuminate Photogels".

Catalog Number

PG95-1

Application

3-D cell culture, bioprinting.

Quantity

Up to 4 mL hydrogel.

Components

Material	Quantity	Concentration
N-Dextran	600 µl	30 mmol/L
HyLink, lyophilized	4 x 200 µl*	10 mmol/L* thiol groups
10x CB (pH 7.2) Phenol Red-free	600 µl	n/a
LAP, 10 mM	600 µl	10 mmol/L
Water	2 x 1500 µl	n.a
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

GP-5: ToLuminate Photogels

Data Sheet

Detailed Product Information

L70-1
200 µl

Shipping Condition: Ambient

77,00 €

3-D Life HyLink

Details

Supporting Information

Description

The ToLuminate HA Photogel kit provides reagents for setting up cell-compatible hydrogels formed by illumination with blue light (365-405 nm) on the basis of the thiol-ene chemistry. Its major components are norbornene-modified dextran (N-Dextran) and the thiol-modified hyaluronic acid crosslinker HyLink. When the two reagents are combined and illuminated in the presence of LAP (Lithium phenyl-2,4,6-trimethylbenzoylphosphinate) thiol groups on HyLink are activated and form stable thioether bonds with norbornene groups on N-Dextran. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. There is ample time to place the pre-gel solution in any culture dish or container, since gelation starts only after illumination. The hydrogel allows cell spreading and migration if cell adhesion peptides are present in the gel.

Cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups on N-Dextran to provide a cell-adhesive matrix.

3-D Life AgaFloat (Cat. No. A10-3) can be added to the pre-gel solution to avoid sedimentation of cells before illumination.

Note: Dextran hydrogels crosslinked with HyLink cannot be dissolved by the addition of dextranase.

For more information and instructions, please consult the General Protocol GP-5 "Preparation of ToLuminate Photogels".

Catalog Number

PG95-1

Application

3-D cell culture, bioprinting.

Quantity

Up to 4 mL hydrogel.

Components

Material	Quantity	Concentration
N-Dextran	600 µl	30 mmol/L
HyLink, lyophilized	4 x 200 µl*	10 mmol/L* thiol groups
10x CB (pH 7.2) Phenol Red-free	600 µl	n/a
LAP, 10 mM	600 µl	10 mmol/L
Water	2 x 1500 µl	n.a
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

GP-5: ToLuminate Photogels

Data Sheet

Detailed Product Information

L70-3
3x 200 µl

Shipping Condition: Ambient

181,00 €

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