3-D Life Dextran-CD Hydrogel SG

Catalog Number: G93-1

Unit Size: 1 Kit

Shipping Temperature: Ambient

205,00 €

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  • Description
  • Specifications

Details
Supporting Information

Description The 3-D Life Dextran-CD Hydrogel SG Kit provides reagents for setting up slow gelling, cell-compatible hydrogels. Its major components are SG-Dextran and the crosslinker CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with thiol-reactive groups on SG-Dextran, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation.

CD-Link contains a matrix metalloproteinase (MMP)-cleavable peptide sequence (Pro-Leu-Gly-Leu-Trp-Ala), which is cleaved by a broad range of MMPs including MMP1, MMP2, MMP3, MMP7, MMP9 and MMP13 [1, 2, 3]. It allows cells to locally degrade the polymer network, if they produce the indicated MMPs.

Prior to the crosslinking step, cell adhesion peptides (e.g. 3-D Life RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups on SG-Dextran to provide a cell-adhesive matrix.

SG-Dextran hydrogels crosslinked with CD-Link can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

For instructions, please consult the General Protocol GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.
Catalog Number G93-1
Application 3-D cell culture, hydrogel injections, filling of microchannels, generation of soft to very stiff gels.
Quantity Allows formation of up to 2 ml 3-D Life Hydrogel depending on the stiffness of the gel.
Components
Material Quantity Concentration
SG-Dextran170 µl30 mmol/L thioreactive groups
CD-Link, lyophilized200 µl*20 mmol/L* thiol groups
10x CB (pH 7.2)200 µln.a.
Water2 x 1500 µln.a
*Volume/concentration after reconstitution of lyophilisate.
Reference[1] Knight, C. G. et al. FEBS 296:263-66 (1992) [2] Patterson, J. and Hubbell, J. A. Biomaterials 31, 7836-7845 (2010) [3] Deng, S. et al. J. Biol. Chem. 275, No. 40, 31422–31427 (2000)

General Protocols

Slow-gelling hydrogels

User Guide

3-D Life Hydrogel User Guide

Data Sheet

Detailed Product Information

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