<span id="hikashop_product_name_main" class="hikashop_product_name_main" itemprop="name"> 3-D Life SG-Dextran

User Guide

Data Sheet

G92-1
1 Kit

Shipping Condition: Ambient

93,00 €

3-D Life Dextran-CD Hydrogel SG

Details

Supporting Information

Description

3-D Life SG-Dextran is a component of the 3-D Life Hydrogel system. It contains dextran functionalized with thiol-reactive groups (SG-Dextran) that can be used together with PEG-Link (Cat. No. L50-1 or L50-3) or CD-Link (Cat. No. L60-1 or L60-3) to set up slow gelling, cell-compatible hydrogels.

When crosslinked with PEG-Link or CD-Link Dextran-based 3-D Life Hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation. Dextran hydrogels crosslinked with HyLink cannot be dissolved by dextranase.

10 x CB (pH 7.2) is a buffer to control pH and osmotic conditions during gel formation in the presence of cells. For more information and instructions, please consult the General Protocol GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

M91-3

Application

3-D cell cultures; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.

Quantity

Without peptide modification:

Up to 12 mL hydrogel at a crosslinking strength of 1.2 mmol/L (used for crosslinking with HyLink or CD-HyLink).
Up to 7.5 mL hydrogel at a crosslinking strength of 2 mmol/L (used for crosslinking with PEG-Link or CD-Link).

The hydrogel volume will be reduced, if peptide modifications are added.

Components

Material	Quantity	Concentration
SG-Dextran	3 x 170 µl	30 mmol/L thioreactive groups
10x CB (pH 7.2)	600 µl	n.a.
Water	4 x 1500 µl	n.a

General Protocols

User Guide

Data Sheet

G93-1
1 Kit

Shipping Condition: Ambient

210,00 €

3-D Life Dextran-HA Hydrogel

Details

Supporting Information

Description

3-D Life SG-Dextran is a component of the 3-D Life Hydrogel system. It contains dextran functionalized with thiol-reactive groups (SG-Dextran) that can be used together with PEG-Link (Cat. No. L50-1 or L50-3) or CD-Link (Cat. No. L60-1 or L60-3) to set up slow gelling, cell-compatible hydrogels.

When crosslinked with PEG-Link or CD-Link Dextran-based 3-D Life Hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation. Dextran hydrogels crosslinked with HyLink cannot be dissolved by dextranase.

10 x CB (pH 7.2) is a buffer to control pH and osmotic conditions during gel formation in the presence of cells. For more information and instructions, please consult the General Protocol GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

M91-3

Application

3-D cell cultures; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.

Quantity

Without peptide modification:

Up to 12 mL hydrogel at a crosslinking strength of 1.2 mmol/L (used for crosslinking with HyLink or CD-HyLink).
Up to 7.5 mL hydrogel at a crosslinking strength of 2 mmol/L (used for crosslinking with PEG-Link or CD-Link).

The hydrogel volume will be reduced, if peptide modifications are added.

Components

Material	Quantity	Concentration
SG-Dextran	3 x 170 µl	30 mmol/L thioreactive groups
10x CB (pH 7.2)	600 µl	n.a.
Water	4 x 1500 µl	n.a

General Protocols

User Guide

Data Sheet

G95-1
1 Kit

Shipping Condition: Ambient

210,00 €

3-D Life PVA-HA Hydrogel

Details

Supporting Information

Description

3-D Life SG-Dextran is a component of the 3-D Life Hydrogel system. It contains dextran functionalized with thiol-reactive groups (SG-Dextran) that can be used together with PEG-Link (Cat. No. L50-1 or L50-3) or CD-Link (Cat. No. L60-1 or L60-3) to set up slow gelling, cell-compatible hydrogels.

When crosslinked with PEG-Link or CD-Link Dextran-based 3-D Life Hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation. Dextran hydrogels crosslinked with HyLink cannot be dissolved by dextranase.

10 x CB (pH 7.2) is a buffer to control pH and osmotic conditions during gel formation in the presence of cells. For more information and instructions, please consult the General Protocol GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

M91-3

Application

3-D cell cultures; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.

Quantity

Without peptide modification:

Up to 12 mL hydrogel at a crosslinking strength of 1.2 mmol/L (used for crosslinking with HyLink or CD-HyLink).
Up to 7.5 mL hydrogel at a crosslinking strength of 2 mmol/L (used for crosslinking with PEG-Link or CD-Link).

The hydrogel volume will be reduced, if peptide modifications are added.

Components

Material	Quantity	Concentration
SG-Dextran	3 x 170 µl	30 mmol/L thioreactive groups
10x CB (pH 7.2)	600 µl	n.a.
Water	4 x 1500 µl	n.a

General Protocols

User Guide

Data Sheet

G85-1
1 Kit

Shipping Condition: Ambient

204,00 €

3-D Life Dextran-CD-HA Hydrogel

Details

Supporting Information

Description

3-D Life SG-Dextran is a component of the 3-D Life Hydrogel system. It contains dextran functionalized with thiol-reactive groups (SG-Dextran) that can be used together with PEG-Link (Cat. No. L50-1 or L50-3) or CD-Link (Cat. No. L60-1 or L60-3) to set up slow gelling, cell-compatible hydrogels.

When crosslinked with PEG-Link or CD-Link Dextran-based 3-D Life Hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation. Dextran hydrogels crosslinked with HyLink cannot be dissolved by dextranase.

10 x CB (pH 7.2) is a buffer to control pH and osmotic conditions during gel formation in the presence of cells. For more information and instructions, please consult the General Protocol GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

M91-3

Application

3-D cell cultures; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.

Quantity

Without peptide modification:

Up to 12 mL hydrogel at a crosslinking strength of 1.2 mmol/L (used for crosslinking with HyLink or CD-HyLink).
Up to 7.5 mL hydrogel at a crosslinking strength of 2 mmol/L (used for crosslinking with PEG-Link or CD-Link).

The hydrogel volume will be reduced, if peptide modifications are added.

Components

Material	Quantity	Concentration
SG-Dextran	3 x 170 µl	30 mmol/L thioreactive groups
10x CB (pH 7.2)	600 µl	n.a.
Water	4 x 1500 µl	n.a

General Protocols

User Guide

Data Sheet