<span id="hikashop_product_name_main" class="hikashop_product_name_main" itemprop="name"> 3-D Life CD-Link

Application Note: Fibroblast Spreading in Cell-Degradable 3-D Life Hydrogels

Applications

Application Protocol: Co-Culture of Tumor and Stroma Cells in 3-D Life Hydrogels

Application Note: Co-culture of tumor and stroma cells in 3-D Life Hydrogels

User Guide

Data Sheet

G93-1
1 Kit

Shipping Condition: Ambient

210,00 €

3-D Life PVA-CD Hydrogel SG

Details

Supporting Information

Description

3-D Life CD-Link is a component of the 3-D Life Hydrogel system. The CD-Link molecule consists of a polyethylene glycol-peptide conjugate carrying a thiol at each end. When combined with polymers of the 3-D Life Hydrogel system, thiol groups on CD-Link form stable thioether bonds with the thiol-reactive groups on the polymers, which results in the formation of the hydrogel. CD-Link can be used with 3-D Life functionalised polymers Mal-PVA, Mal-Dextran, SG-PVA and SG-Dextran.

CD-Link contains a matrix metalloproteinase (MMP)-cleavable peptide sequence (Pro-Leu-Gly-Leu-Trp-Ala), which is cleaved by a broad range of MMPs including MMP1, MMP2, MMP3, MMP7, MMP9 and MMP13 [1, 2, 3]. It allows cells to locally degrade the polymer network, if they produce the indicated MMPs.

For instructions, please consult General Protocols GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" or GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

L60-3

Application

3-D Life CD-Link is used together with thiol-reactive polymers of the 3-D Life Hydrogel system to establish 3-D cell cultures.

Quantity

When used with polymers of the 3-D Life Hydrogel system up to 6 ml 3-D Life Hydrogel can be generated, depending on the stiffness of the gel.

Components

Material	Quantity	Concentration
CD-Link, lyophilized	3 x 200 µl*	20 mmol/L* thiol groups
Water	600 µl	n/a
*Volume/concentration after reconstitution of lyophilisate.

Reference

[1] Knight, C. G. et al. FEBS 296:263-66 (1992) [2] Patterson, J. and Hubbell, J. A. Biomaterials 31, 7836-7845 (2010) [3] Deng, S. et al. J. Biol. Chem. 275, No. 40, 31422–31427 (2000)

General Protocols

Application Note: Fibroblast Spreading in Cell-Degradable 3-D Life Hydrogels

Applications

Application Protocol: Co-Culture of Tumor and Stroma Cells in 3-D Life Hydrogels

Application Note: Co-culture of tumor and stroma cells in 3-D Life Hydrogels

User Guide

Data Sheet

G83-1
1 Kit

Shipping Condition: Ambient

204,00 €

3-D Life Dextran-CD Hydrogel FG

Details

Supporting Information

Description

3-D Life CD-Link is a component of the 3-D Life Hydrogel system. The CD-Link molecule consists of a polyethylene glycol-peptide conjugate carrying a thiol at each end. When combined with polymers of the 3-D Life Hydrogel system, thiol groups on CD-Link form stable thioether bonds with the thiol-reactive groups on the polymers, which results in the formation of the hydrogel. CD-Link can be used with 3-D Life functionalised polymers Mal-PVA, Mal-Dextran, SG-PVA and SG-Dextran.

CD-Link contains a matrix metalloproteinase (MMP)-cleavable peptide sequence (Pro-Leu-Gly-Leu-Trp-Ala), which is cleaved by a broad range of MMPs including MMP1, MMP2, MMP3, MMP7, MMP9 and MMP13 [1, 2, 3]. It allows cells to locally degrade the polymer network, if they produce the indicated MMPs.

For instructions, please consult General Protocols GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" or GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

L60-3

Application

3-D Life CD-Link is used together with thiol-reactive polymers of the 3-D Life Hydrogel system to establish 3-D cell cultures.

Quantity

When used with polymers of the 3-D Life Hydrogel system up to 6 ml 3-D Life Hydrogel can be generated, depending on the stiffness of the gel.

Components

Material	Quantity	Concentration
CD-Link, lyophilized	3 x 200 µl*	20 mmol/L* thiol groups
Water	600 µl	n/a
*Volume/concentration after reconstitution of lyophilisate.

Reference

[1] Knight, C. G. et al. FEBS 296:263-66 (1992) [2] Patterson, J. and Hubbell, J. A. Biomaterials 31, 7836-7845 (2010) [3] Deng, S. et al. J. Biol. Chem. 275, No. 40, 31422–31427 (2000)

General Protocols

Application Note: Fibroblast Spreading in Cell-Degradable 3-D Life Hydrogels

Applications

Application Protocol: Co-Culture of Tumor and Stroma Cells in 3-D Life Hydrogels

Application Note: Co-culture of tumor and stroma cells in 3-D Life Hydrogels

User Guide

Data Sheet

FG91-1
1 Kit

Shipping Condition: Ambient

210,00 €

3-D Life PVA-CD Hydrogel FG

Details

Supporting Information

Description

3-D Life CD-Link is a component of the 3-D Life Hydrogel system. The CD-Link molecule consists of a polyethylene glycol-peptide conjugate carrying a thiol at each end. When combined with polymers of the 3-D Life Hydrogel system, thiol groups on CD-Link form stable thioether bonds with the thiol-reactive groups on the polymers, which results in the formation of the hydrogel. CD-Link can be used with 3-D Life functionalised polymers Mal-PVA, Mal-Dextran, SG-PVA and SG-Dextran.

CD-Link contains a matrix metalloproteinase (MMP)-cleavable peptide sequence (Pro-Leu-Gly-Leu-Trp-Ala), which is cleaved by a broad range of MMPs including MMP1, MMP2, MMP3, MMP7, MMP9 and MMP13 [1, 2, 3]. It allows cells to locally degrade the polymer network, if they produce the indicated MMPs.

For instructions, please consult General Protocols GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" or GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

L60-3

Application

3-D Life CD-Link is used together with thiol-reactive polymers of the 3-D Life Hydrogel system to establish 3-D cell cultures.

Quantity

When used with polymers of the 3-D Life Hydrogel system up to 6 ml 3-D Life Hydrogel can be generated, depending on the stiffness of the gel.

Components

Material	Quantity	Concentration
CD-Link, lyophilized	3 x 200 µl*	20 mmol/L* thiol groups
Water	600 µl	n/a
*Volume/concentration after reconstitution of lyophilisate.

Reference

[1] Knight, C. G. et al. FEBS 296:263-66 (1992) [2] Patterson, J. and Hubbell, J. A. Biomaterials 31, 7836-7845 (2010) [3] Deng, S. et al. J. Biol. Chem. 275, No. 40, 31422–31427 (2000)

General Protocols

Application Note: Fibroblast Spreading in Cell-Degradable 3-D Life Hydrogels

Applications

Application Protocol: Co-Culture of Tumor and Stroma Cells in 3-D Life Hydrogels

Application Note: Co-culture of tumor and stroma cells in 3-D Life Hydrogels

User Guide

Data Sheet

FG81-1
1 Kit

Shipping Condition: Dry ice

204,00 €

3-D Life CD-Link

Details

Supporting Information

Description

3-D Life CD-Link is a component of the 3-D Life Hydrogel system. The CD-Link molecule consists of a polyethylene glycol-peptide conjugate carrying a thiol at each end. When combined with polymers of the 3-D Life Hydrogel system, thiol groups on CD-Link form stable thioether bonds with the thiol-reactive groups on the polymers, which results in the formation of the hydrogel. CD-Link can be used with 3-D Life functionalised polymers Mal-PVA, Mal-Dextran, SG-PVA and SG-Dextran.

CD-Link contains a matrix metalloproteinase (MMP)-cleavable peptide sequence (Pro-Leu-Gly-Leu-Trp-Ala), which is cleaved by a broad range of MMPs including MMP1, MMP2, MMP3, MMP7, MMP9 and MMP13 [1, 2, 3]. It allows cells to locally degrade the polymer network, if they produce the indicated MMPs.

For instructions, please consult General Protocols GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" or GP-2 "Preparation of 3-D Life Slow Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.

Catalog Number

L60-3

Application

3-D Life CD-Link is used together with thiol-reactive polymers of the 3-D Life Hydrogel system to establish 3-D cell cultures.

Quantity

When used with polymers of the 3-D Life Hydrogel system up to 6 ml 3-D Life Hydrogel can be generated, depending on the stiffness of the gel.

Components

Material	Quantity	Concentration
CD-Link, lyophilized	3 x 200 µl*	20 mmol/L* thiol groups
Water	600 µl	n/a
*Volume/concentration after reconstitution of lyophilisate.

Reference

[1] Knight, C. G. et al. FEBS 296:263-66 (1992) [2] Patterson, J. and Hubbell, J. A. Biomaterials 31, 7836-7845 (2010) [3] Deng, S. et al. J. Biol. Chem. 275, No. 40, 31422–31427 (2000)

General Protocols

Application Note: Fibroblast Spreading in Cell-Degradable 3-D Life Hydrogels

Applications

Application Protocol: Co-Culture of Tumor and Stroma Cells in 3-D Life Hydrogels

Application Note: Co-culture of tumor and stroma cells in 3-D Life Hydrogels

User Guide

Data Sheet