3-D Life ToGro Hydrogel

Catalog Number: G94-1

Unit Size: 1 Kit

Shipping Temperature: Ambient

Price: 279,00 €

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Description
Specifications

Details

Supporting Information

Description

The 3-D Life ToGro Hydrogel Kit contains reagents for the preparation of hydrogels with a soft stiffness (400 Pa; shear modulus) and a RGD Peptide concentration of 0.4 mmol/L. The gel composition allows three-dimensional spreading and migration of most cells. The gel is formed by the crosslinking of modified dextran carrying the cell adhesion motif RGD (RGD-Dextran) with CD-Link. When the two reagents are combined, thiol groups on CD-Link form stable thioether bonds with thiol-reactive groups on RGD-Dextran resulting in gel formation within 20 min. The formation of hydrogel is performed at physiological pH for optimal cell compatibility. CD-Link is composed of polyethylene glycol and a matrix metalloprotease (MMP)-cleavable peptide. The MMP-cleavable peptide is designed for a broad range of MMP cleavage including MMPs MMP1, MMP3, MMP7 and MMP9 [1]. It allows cells to spread and migrate within the gel if they express the indicated MMPs. 3-D Life ToGro hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase Cat. No. D10-1), which allows recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or further cultivation. For preparation of gels follow the instructions of the 3-D Life ToGro Protocol GP-3 “Preparation of 3-D Life ToGro Hydrogel”. The document can be downloaded.

Catalog Number

G94-1

Application

3-dimensional cultivation of various cell types, spheroids, tissue samples and similar requiring a cell-adhesive and cell-degradable matrix for cell growth and differentiation.

Quantity

Allows formation of 2.4 ml 3-D Life ToGro Hydrogel.

Components

Material	Quantity	Concentration
RGD-Dextran, lyophilized	2 x 870 µl*	n.a.	MSDS
CD-Link, lyophilized	200 µl*	20 mmol/L* thiol groups	MSDS
Reconstitution Buffer	2 x 900 µl	n.a	MSDS
Water	600 µl	n/a	MSDS
*Volume/concentration after reconstitution of lyophilisate.

General Protocols

Preconfigured ToGro Hydrogel

Data Sheet

Detailed Product Information

3-D Life ToGro Hydrogel

General Protocols

Data Sheet

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