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3-D Life
Dextran-PEG Hydrogel SG
Catalog Number: G92-1
Unit Size: 1 Kit
Shipping Temperature: Ambient
Price:
89,00 €
How to Order
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Description
Specifications
Details
Supporting Information
Description
The
3-D Life
Dextran-PEG Hydrogel SG Kit provides reagents for setting up slow gelling, cell-compatible hydrogels. Its major components are SG-Dextran and the crosslinker PEG-Link. When the two reagents are combined, thiol groups on PEG-Link form stable thioether bonds with thiol-reactive groups on SG-Dextran, which results in the formation of the gel. The components are mixed at physiological pH (pH 7.2) for optimal cell compatibility. The slow gelation kinetics allows enough time to conveniently manipulate the solution before the onset of gel formation. Prior to the crosslinking step, cell adhesion peptides (e.g.
3-D Life
RGD Peptide, Cat. No. 09-P-001) can be covalently attached to a portion of the SH-reactive groups on SG-Dextran to provide a cell-adhesive matrix. Dextran hydrogels can be dissolved by the addition of dextranase (
3-D Life
Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.For more information and instructions, please consult the General Protocol GP-2 "Preparation of
3-D Life
Slow Gelling Hydrogels" and the
3-D Life
Hydrogels User Guide.
Catalog Number
G92-1
Application
3-D cell culture, hydrogel injections, filling of microchannels, generation of soft to very stiff gels.
Quantity
Allows formation of up to 2 ml
3-D Life
Hydrogel depending on the stiffness of the gel.
Components
Material
Quantity
Concentration
SG-Dextran
170 µl
30 mmol/L thioreactive groups
MSDS
PEG-Link, lyophilized
200 µl*
20 mmol/L* thiol groups
MSDS
10x CB (pH 7.2)
200 µl
n.a.
MSDS
Water
2 x 1500 µl
n.a
MSDS
*Volume/concentration after reconstitution of lyophilisate.
General Protocols
Slow-gelling hydrogels
User Guide
3-D Life
Hydrogel User Guide
Data Sheet
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