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3-D Life
CD-Link
Catalog Number: L60-1
Unit Size: 200 µl
Shipping Temperature: Ambient
Price:
150,00 €
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Description
Specifications
Details
Supporting Information
Description
3-D Life
CD-Link is a component of the
3-D Life
Hydrogel system. The CD-Link molecule consists of a polyethylene glycol-peptide conjugate carrying a thiol at each end. When combined with polymers of the
3-D Life
Hydrogel system, thiol groups on CD-Link form stable thioether bonds with the thiol-reactive groups on the polymers, which results in the formation of the hydrogel. CD-Link can be used with
3-D Life
functionalised polymers Mal-PVA, Mal-Dextran, SG-PVA and SG-Dextran. CD-Link contains a matrix metalloproteinase (MMP)-cleavable peptide sequence (Pro-Leu-Gly-Leu-Trp-Ala), which is cleaved by a broad range of MMPs including MMP1, MMP3, MMP7 and MMP9 [1]. It allows cells to locally degrade the polymer network, if they produce the indicated MMPs.For instructions, please consult General Protocols GP-1 "Preparation of
3-D Life
Fast Gelling Hydrogels" or GP-2 "Preparation of
3-D Life
Slow Gelling Hydrogels" and the
3-D Life
Hydrogels User Guide.
Catalog Number
L60-1
Application
3-D Life
CD-Link is used together with thiol-reactive polymers of the
3-D Life
Hydrogel system to establish 3-D cell cultures.
Quantity
When used with polymers of the
3-D Life
Hydrogel system up to 2 ml
3-D Life
Hydrogel can be generated, depending on the stiffness of the gel.
Components
Material
Quantity
Concentration
CD-Link, lyophilized
200 µl*
20 mmol/L* thiol groups
MSDS
Water
600 µl
n/a
MSDS
*Volume/concentration after reconstitution of lyophilisate.
Reference
[1] Knight, C. G. et al. FEBS 296:263-66 (1992)
General Protocols
Slow-gelling hydrogels
Fast-gelling Hydrogels
Preconfigured ToGro Hydrogel
Applications
Application Note: Fibroblast Spreading in Cell-Degradable 3-D Life Hydrogels
Application Protocol: Co-Culture of Tumor and Stroma Cells in 3-D Life Hydrogels
Application Note: Co-culture of tumor and stroma cells in 3-D Life Hydrogels
User Guide
3-D Life
Hydrogel User Guide
Data Sheet
Detailed Product Information
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