The authors took an innovative approach and used the flexibility of the 3-D Life Hydrogel system to design a clustered RGD Peptide microenvironment to study patterns of cell adhesion ligands on cell behavior.
Publications
Wang et al. (2019) Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions. Biomaterials 35(10): 3273–3280. (Article) Applications
Collective cell behavior, hydrogel tailoring
Methods
Live/Dead staining (Calcein AM/PI), confocal fluorescence microscopy, nuclear (DAPI) staining, phalloidin staining
Cells
3T3 (fibroblast, murine), C2C12 (myoblasts, murine)
Products used
3-D Life Dextran-CD Hydrogel FG
3-D Life RGD Peptide
3-D Life Dextranase
3-D Life Thioglycerol
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