The 3-D Life Hydrogel system was used to generate hydrogels with controlled variations in local stiffness at microscale dimensions to examine mechanotransductional effects on human mesenchymal stem cells.Publications
Wang et al. (2021) Spatial micro-variation of 3D hydrogel stiffness regulates the biomechanical properties of hMSCs. Biofabrication 13: 035051 (Article) Applications
Mechanotransduction
Methods
Atomic force microscopy, rheology, Live/Dead staining (Calcein AM/PI), immunocytochemistry, paraffin embedment and sectioning, hematoxylin-eosin staining
Cells
HMSCs (human mesenchymal stem cells)
Products used
3-D Life Dextran-CD Hydrogel FG
3-D Life RGD Peptide
3-D Life Thioglycerol
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