3-D Life Hydrogel was used to create an extracellular matrix-lumen interface within a microfluidic chip. The successful co-culture of tumor spheroids in the gel and the epithelial cell layer on the gel surface shows the suitability of the setup as a cellular barrier model.Publications
Nair et al. (2021) Parallelizable Microfluidic Platform to Model and Assess In Vitro Cellular Barriers: Technology and Application to Study the Interaction of 3D Tumor Spheroids with Cellular Barriers. Biosensors 11(9), 314 (Article) Applications
Epithelial barrier model, transepithelial cell migration
Methods
Hydrogel in a microfluidic platform, transepithelial electrical resistance (TEER)
Cells
MDCK (kidney epithelial, canine), HT29 (colon adenocarcinoma, human)
Products used
3-D Life PVA-CD Hydrogel SG
3-D Life RGD Peptide
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