The work shows how different stiffnesses of 3-D Life Hydrogels can direct the differentiation of endothelial progenitor cells (EPCs) into a venous or arterial phenotype. The gels injected into nude mice support the in vivo formation of functional blood vessels.
Publications
Xue et al. (2019) Matrix stiffness regulates arteriovenous differentiation of endothelial progenitor cells during vasculogenesis in nude mice. Cell Proliferation 52:e12557 (Article) Applications
Angiogenesis, vasculogenesis
Methods
Paraffin embedment and sectioning, hydrogel injection into mice, antibody staining of tissue slices, dissolution of gels with dextranase and subsequent analysis of cells with qPCR and Western blotting
Cells
EPCs (endothelial progenitor cells, murine)
Products used
3-D Life Dextran-CD Hydrogel FG
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