Molecular mechanisms of de novo epithelial lumen formation is studied in long term cultures of MCF10A mammary epithelial cells in 3-D Life Hydrogels.Publications
Grikscheit, K. et al. (2015) Junctional actin assembly is mediated by Formin-like 2 downstream of Rac 1. J. Cell Biol. 209:367-76. (PubMed) Applications
Epithelial junction formation
Methods
Fluorescence microscopy, nuclear (DAPI) staining, phalloidin staining
Cells
MCF-10A (mammary breast epithelial, non tumorigenic, human)
Products used
3-D Life Dextran-PEG Hydrogel FG
3-D Life RGD Peptide
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