Using the 3-D Life Hydrogel system a reproducible tumor-stroma model including macrophages, neutrophils and fibroblasts within a malignant tumor microenvironment was developed. In contrast to collagen-based matrices, where the matrix strongly contracts during a long culture period, the 3-D Life Hydrogel maintained its size.
Publications
Hensler et al. (2021) A novel standardized inflammatory cell-modulated 3D tumor tissue model for analysis of tumor-stroma interaction and drug discovery. American Journal of Bioscience and Bioengineering 9(4): 110-122 (Link) Applications
Tumor-stroma model, drug testing
Methods
Hydrogel culture in transwell 24 well plates, on-top culture, air-liquid-interface (ALI)
Cells
HaCaT (human keratinocytes), H838 (human non-small-cell lung carcinoma), HL-60 (human, differentiated to neutrophils), U937 (human lymphoblast), MCF-7 (mamma carcinoma, human)
Products used
3-D Life RGD Peptide
3-D Life Dextran-CD Hydrogel SG
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