Cartilage intermediate layer protein (CILP) and aggrecan and collagen II synthesis were measured in human nucleus pulposus (NP) cells in response to mechanical stimuli, including cyclic compressive stress and cyclic tensile strain. Publications
He et al. (2018) Cartilage intermediate layer protein is regulated by mechanical stress and affects extracellular matrix synthesis. Molecular Medicine Reports 17: 6130-6137 (Article) Applications
Cartilage research
Methods
Compression loading, dissolution of gels with dextranase and subsequent analysis of cells with qPCR and Western blotting, mechanical stress application
Cells
Nucleus pulposus cells (human)
Products used
3-D Life Dextran-PEG Hydrogel FG
3-D Life Dextranase
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