3-D Life Hydrogel is used to culture human pancreas organoids (hPOs) in a chemically definded 3D matrix. Isolated pancreatic ducts formed organoids in 3-D Life Hydrogel comparable to the ones cultured in basement membrane extracts (BME 2). Using a newly developed culture medium which specifically supports the growth of human pancreatic organoids, hPOs were expanded and passaged several times using 3-D Life Dextranase. hPOs expressed similar levels of the progenitor and beta cell marker PDX1 and, although at lower levels, the ductal markers KRT19 and SOX9 when compared with hPOs cultured in BME 2. In conclusion, hPOs well maintain a pancreatic ductal identity when cultured in 3-D Life Hydrogel.Publications
Georgakopoulos et al. (2020) Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids. BMC Developmental Biology 20:4 (Article) Applications
Organoid culture
Methods
Immunofluorescence staining, confocal fluorescence microscopy, Hoechst staining, recovery of cells from gels by dextranase treatment and subsequent qRT-PCR
Cells
Pancreatic progenitor cells (human)
Products used
3-D Life HyLink
3-D Life SG-Dextran
3-D Life RGD Peptide
3-D Life Dextranase
3-D Life Dextran-HA Hydrogel
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