Co-culture of HUVECs and fibroblasts in 3-D Life Hydrogels to assess titanium-hydrogel-cell compatibility for future implantation strategies.Publications
Koenig, G., et al. (2016) Cell-laden hydrogel/titanium microhybrids: Site-specific cell delivery to metallic implants for improved integration. Acta Biomater. 2016 Mar;33:301-10. (link) Applications
Implantation, biocompatibility studies, titanium-hydrogel implant development
Methods
Fluorescence microscopy, scanning electron microscopy of hydrogels, Alamar Blue Assay, Hoechst staining, phalloidin staining, formaldehyde fixation, recovery of cells from gels by dextranase treatment and subsequent qRT-PCR , Calcein AM (cell viability assay), glutaraldehyde fixation, live cell tracking
Cells
3T3 (fibroblast, murine), HUVECs (Human Umbilical Vein Endothelial Cells)
Products used
3-D Life PVA-CD Hydrogel FG
3-D Life Dextran-PEG Hydrogel FG
3-D Life Dextran-CD Hydrogel FG
3-D Life RGD Peptide
3-D Life Dextranase
3-D Life Thioglycerol
3-D Life PVA-PEG Hydrogel FG
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