3-D Life hydrogel supplemented with RGD Peptide was used for long term culture (14 days) of human breast epithelial cells (MCF10A). Successful lumen formation by spheroids was observed with wildtype cells and investigated with mutated cells in this type of cultures.Publications
Grobe, H. et al. (2018) A Rac1-FMNL2 signaling module affects cell-cell contact formation independent of Cdc42 and membrane protrusions. PLoS One 13:e0194716 (Article) Applications
Epithelial junction formation
Methods
Confocal fluorescence microscopy, nuclear (DAPI) staining, phalloidin staining, formaldehyde fixation
Cells
MCF-10A (mammary breast epithelial, non tumorigenic, human)
Products used
3-D Life Dextran-PEG Hydrogel FG
3-D Life RGD Peptide
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