A novel peptide modification of 3-D Life Hydrogel was used to establish a neurosphere outgrowth assay for developmental neurotoxicity compound testing. The work shows how cell migration, differentiation to neurons and formation of neuronal networks is supported by the hydrogel. Publications
Hellwig, C. et al. (2018) Culture of human neurospheres in 3D scaffolds for developmental neurotoxicity testing. Toxicology in vitro 52:106-115 (Link) Applications
Drug testing, neurotoxicity
Methods
Rheology, neurosphere outgrowth, Alamar Blue Assay, fluorescence microscopy, immunocytochemistry, Hoechst staining, phalloidin staining
Cells
Neurospheres (human)
Products used
3-D Life FG-PVA
3-D Life 10x CB Buffer (pH 7.2)
3-D Life CD-Link
3-D Life RGD Peptide
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