The effect of multiaxial cell stretch on adult ventricular cardiomyocytes (CM) embedded in 3-D Life Cellendes hydrogel of different stiffnesses (Young modulus of 1 kPa, 4-9 kPa and > than 10 kPa) was examined on a cell stretch system (IsoStretcher). Single CMs were embedded in SG-PVA-PEG hydrogels modified with RGD Peptide to allow for adhesion of cells to the surrounding hydrogel. Stretch-induced Ca2+ influx was measured with the Ca2+ indicator Fluo-4 AM which was mixed into the hydrogel before gelation.Publications
Friedrich, O. et al. (2017) Adding dimension to cellular
mechanotransduction: Advances in biomedical engineering of multiaxial cell-stretch systems and
their application to cardiovascular biomechanics and mechano-signaling. Progress in Biophysics and Molecular Biology 130:170-191 (Link) Applications
Mechanotransduction, mechanobiology, cardiac research
Methods
Ca2+ influx measurement with Fluo-4 AM , cell stretching
Cells
Cardiomyocytes (murine)
Products used
3-D Life PVA-PEG Hydrogel SG
3-D Life RGD Peptide
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