The 3-D Life Hydrogel technology was used to prepare maleimide-modified serum albumin (HSA) which was crosslinked with PEG-Link and supplemented with 4% hyaluronan (HSA–HA) for the cultivation of mesenchymal stem cells, disk cells and chondrocytes. The HSA–HA hydrogel turned out to be a suitable biomaterial for the cultivation and/or differentiation of chondrogenic cells.Publications
Benz, K., et al. (2010) Polyethylene Glycol-Crosslinked Serum Albumin/Hyaluronan Hydrogel for the Cultivation of Chondrogenic Cell Types. A Adv. Eng. Mater. 12:B539-B551
(link) Applications
Chondrogenesis, tissue regeneration
Methods
Live/Dead staining (Calcein AM/PI), recovery of cells from serum albumin/PEG hydrogel by digestion with proteinase K and subsequent qRT-PCR, hydrogel preparation through a twin-chamber syringe, cell proliferation asay (PicoGreen)
Cells
HMSCs (human mesenchymal stem cells), Intervertebral disk cells (human), Chondrocytes, Human chondrocytes
Products used
serum albumin/PEG hydrogel, available upon request
3-D Life PEG-Link
Please send a message to order products or if you would like to know more about Cellendes:
Cellendes GmbH
Gerhard-Kindler-Str. 8
D-72770 Reutlingen
Germany
Ph.: +49 7121 15940 0
Fax: +49 7121 15940 99
info@cellendes.com