3-D Life Hydrogel is used to develop an artificial lymph node model using a perfused bioreactor system. PBMCs, antigen-presenting dendritic cells, and MSC-derived stromal cells are co-cultivated.Publications
Sardi, M. et al. (2016) Modeling Human Immunity In Vitro: Improving artificial lymph node physiology by stromal cells. Appl Vitr Toxicol. 2016:2(3);143-150. (link) Applications
Lymph node model
Methods
Immunofluorescence staining, confocal fluorescence microscopy, perfusion, nuclear (DAPI) staining, formaldehyde fixation, bioreactor system
Cells
APCs (antigen presenting cells), PBMCs (human peripheral blood mononuclear cells), MCF-7 (mamma carcinoma, human), UC-MSC (umbilical cord mesenchymal stem cells), ADSCs (adipose-derived stem cells), BM-MSC (bone marrow mesenchymal stem cells), ATSVF (Adipose tissue stromal vascular fraction)
Products used
3-D Life Dextran-CD Hydrogel SG
3-D Life RGD Peptide
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