<em>3-D Life</em> FG-Dextran

3-D Life FG-Dextran

‎€155.00
SKU: M92-3

Cat. No. : M92-3


3-D Life FG-Dextran is a component of the 3-D Life Hydrogel system. It contains maleimide-functionalized dextran (Mal-Dextran) that can be used together with PEG-Link (Cat. No.L50-1 or L50-3) or CD-Link (Cat. No. L60-1 or L60-3) to set up fast-gelling biomimetic hydrogels for 3-D cell culture.





Description 3-D Life FG-Dextran is a component of the 3-D Life Hydrogel system. It contains maleimide-functionalized dextran (Mal-Dextran) that can be used together with PEG-Link (Cat. No.L50-1 or L50-3) or CD-Link (Cat. No. L60-1 or L60-3) to set up fast gelling, cell-compatible hydrogels.

Dextran-based 3-D Life hydrogels can be dissolved by the addition of dextranase (3-D Life Dextranase, Cat. No. D10-1), which allows the recovery of chemically fixed or live cells for post-culture analyses (e.g. RT-PCR) or for further cultivation.

10 x CB (pH 7.2) is a buffer to control pH and osmotic conditions during gel formation in the presence of cells.

For instructions, please consult the General Protocol GP-1 "Preparation of 3-D Life Fast Gelling Hydrogels" and the 3-D Life Hydrogels User Guide.
Application 3-D cell cultures; embedment and immobilization of cells or tissues and subsequent recovery of specimens by hydrogel dissolution.
Quantity Allows formation of up to 6 ml 3-D Life Hydrogel depending on the stiffness of the gel, if used together with thiol-functionalized polymers (3-D Life PEG-Link or CD-Link).
Components
Material Quantity Concentration of reactive groups
Mal-Dextran, lyophilized3 x 170 µl*30 mmol/L*MSDS
10x CB (pH 5.5)600 µln.a.MSDS
Water4 x 1500 µln.aMSDS
*Volume/concentration after reconstitution of lyophilisate.