Cell Lines and Primary Cells used in 3-D Life Hydrogels



Cell Type Application References
Cell Lines
U937 (lymphoblast)
HL-60 (promyelocytic)
HaCaT (keratinocyte)
MFC-7 (mamma carcinoma, human)
H838 (lung carcinoma)
Tumor-stroma modelHensler et al., 2021
MCF-7 (mamma carcinoma, human)
3T3 (fibroblasts, murine)
Tumor-stroma modelWang et al., 2021
Charwat, V. et al., 2015
HT29 (colon adenocarcinoma, human)
MDCK (kidney epithelial, canine)
Epithelial barrier model; microfluidicsNair et al., 2021
OCI-AML3 (leukemic)Migration assayZippel et al., 2019
HepG2 (hepatocyte carcinoma, human)Spheroid cultureMiyakawa et al., 2018
HepG2 (hepatocyte carcinoma, human)
Huh-7 (hepatocyte carcinoma, human)
MechanotransductionShen et al., 2019
MCF10A (mammary breast epithelial, non tumorigenic, human)Cancer researchGrobe, H. et al., 2018
Grikscheit, K. et al., 2015
U87 (glioblastoma, human)Cancer research, chemotaxis, cell migrationHuang et al., 2018
HKC-8 (kidney proximal tubular, human)
PU009-F (renal fibroblasts, human)
Kidney fibrosis modelNugraha et al., 2017
KCOT (keratocystic odontogenic tumor, human)
Cancer research, spheroid cultureNoguchi et al., 2017
3T3 (fibroblast, murine)Tisssue engineeringKoenig, G., et al., 2016
HCT-116 (colon carcinoma, human)Drug screeningRimann, M., Graf-Hausner, U., 2012
HeLa (adenocarcinoma, human)
HT1080 (epithelial cancer cell line from fibrosarcoma, human)
MDA-MB-231 (mamma carcinoma, human
NES (neuroepithelial-like stem cell derived from H9 hESC, human)
MicroarrayUeda, E., et al., 2012
C2C12 (myoblasts, murine)Cell-matrix interactions; mechanotransductionYin et al., 2021
Wang et al., 2019
Primary Cells
Fibroblasts (human)Tumor-stroma modelHensler et al., 2021
hMSCs (human mesenchymal stem cells)MechanotransductionWang et al., 2021
Benz, K., et al., 2010
ChondrocytesTissue engineering; cell-based therapeutic developmentLang et al., 2021
Rothdiener M. et al., 2018
Benz, K., et al., 2010
Pancreatic progenitor cells (human)Organoid cultureGeorgakopoulos et al., 2020
Cardiomyocytes (murine)MechanotransductionFriedrich et al., 2019
Friedrich, O. et al., 2017
PBMCs (human peripheral blood mononuclear cells)Lymph node model; perfusionKraus et al., 2019
APCs (antigen presenting cells)
DCs (dentritic cells)
ATSVF (Adipose tissue stromal vascular fraction)
BM-MSC (bone marrow mesenchymal stem cells)
ADSCs (adipose-derived stem cells)
UC-MSC (umbilical cord mesenchymal stem cells)
Lymph node model; perfusionSardi, M. et al., 2016
EPCs (endothelial progenitor cells, murine)Matrix-stiffness-dependent cell differentiation; hydrogel implantation in miceXue et al., 2019
Neurospheres (human)Neurotoxicity testingHellwig, C. et al., 2018
Nucleus pulposus (human)MechanotransductionHe et al., 2018
Intervertebral disk cells (human)Cell-based therapeutic developmentBenz, K., et al., 2010
Pancreatic islets (murine)Cell-based therapeutic development; implantationAyenehdeh et al., 2017
AT-MSCs (adipose tissue derived mesenchymal stem cells, murine)Cell-based therapeutic development; implantationAyenehdeh et al., 2017
HUVECs (Human Umbilical Vein Endothelial Cells)

Cell-based therapeutic development, implantation;angiogenesisKoenig, G., et al., 2016
Sun, J. et al., 2014
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