Publications

3-D Life Hydrogel was dispensed in 150 nl droplets on 672 spots in a microarray format (Droplet Microarray, DMA, Aquarray GmbH). In one approach 50 cells were cultured in each hydrogel droplet. In another approach spheroids were formed in culture medium with the hanging drop method on the microarray slide and subsequently immobilized in hydrogel by injection of hydrogel pregel solution into the droplets. The DMA technology combined with automated 3-D Life Hydrogel dispension and image analysis on the microarray allows for HTS applications such as drug screening, migration and invasion assays while saving reagents and compounds in 2-3 orders of magnitude compared to 96- and 384-well plates.