Human breast cancer cells were embedded in 3-D Life Hydrogel on a novel microfluidic system to assess its applicability in investigating CAR-T cell mediated therapeutic treatments. The publication shows how CAR-T cells can migrate into the hydrogel towards the embedded tumor cell aggregates and how subsequent cytokine release can be measured. It is also shown how the chip-based hydrogel cell culture can be used to assess treatments for antagonizing the Cytokine Release Syndrome.
Publications
Maulana et al. (2024) Solid tumor-on-chip model for efficacy and safety assessment of CAR-T cell therapy. Cell Stem Cell 31:1-14 (Article) Applications
Tumor-on-chip model, tumor infiltration, cell extravasation, drug screening, drug testing, cytokine release
Methods
Injection of 3-D Life Hydrogel into microchannels, fluorescence microscopy
Cells
MDA-MB-231 (human breast cancer cell line), Fibroblasts (human), T cells, Human breast cancer organoids (patient derived) , CAR-T cells
Products used
3-D Life RGD Peptide
3-D Life Dextran-CD Hydrogel SG
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