The 3-D Life Hydrogel system was used in a microfluidic platform specifically developed for the 3-D co-cultivation of human cervical cancerous tissue (spheroids) with donor-derived cervical fibroblasts. Donor-derived neutrophils were integrated in the microfluidic system and were recruited into the hydrogel-based co-culture. The 3-D Life hydrogel demonstrated good stability without shrinkage and provided a robust 3D matrix for the cells. The treatment of the culture with cisplatin demonstrates the applicability of the platform for drug testing and the development of new (immuno) therapeutic options.Publications
Kromidas et al. (2023) Immunocompetent PDMS-Free Organ-on-Chip Model of Cervical Cancer Integrating Patient-Specific Cervical Fibroblasts and Neutrophils. Adv. Healthcare Mater.:2302714 (Link) Applications
Drug screening, Organ-on-Chip, Microphysiological System (MPS), tumor-stroma model, cell-based therapeutic development, drug testing, tumor-on-chip model, microfluidics, cancer research, cell migration
Methods
Fluorescence microscopy, Live/Dead staining (Calcein AM/PI), immunofluorescence staining, LDH assay (cytotoxicity), TUNEL Assay, microphysiological system, perfusion, KI-67 staining, hydrogel in a microfluidic platform, fluorescence imaging, drug administration, Sytox Green Assay (nuclear staining)
Cells
PMNs (human polymorphonuclear leukocytes, neutrophils, from whole blood), PBMCs (human peripheral blood mononuclear cells), SiHa (human squamous cervical cancer cell line)
, Fibroblasts (from human cervix uteri)
Products used
3-D Life SG-Dextran
3-D Life RGD Peptide
3-D Life CD-HyLink
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