Automated drug screening of tumor spheroids in 3-D Life Hydrogels. Demonstrates the different drug sensitvities of tumor cells in 2-D versus 3-D cell culture.Publications
Rimann, M. et al. (2014) Automation of 3D Cell Culture Using Chemically Defined Hydrogels. J. Lab. Autom. 19:191-197
(link) Applications
Drug screening, automation
Methods
Phase-contrast microscopy, robotic liquid handling, cell viability (ATP assay), recovery of cells by dextranase treatment and subsequent DNA content quantification
Cells
HCT 116 (colon carcinoma, human)
Products used
3-D Life Dextran-PEG Hydrogel FG
3-D Life RGD Peptide
3-D Life Dextranase
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