Normal and injured primary human skeletal muscle cells (HSkMCs) were embedded in 3-D Life Hydrogel and submitted to rolling manipulation, a specific mechanical stimulation used in chinese medicine, to assess its effect on injured skeletal muscle cells. After hydrogel cultures were submitted during a 3 day cultivation to rolling manipulation, cells were recovered from the hydrogels using Dextranase and subsequently treated for protein extraction. Proteomic data and Western Blot analysis showed which signalling pathways might be important pathways by which rolling manipulation ameliorates HSkMC injury.Publications
Zhao et al. (2021) Intermittent pressure imitating rolling manipulation ameliorates injury in
skeletal muscle cells through oxidative stress and lipid metabolism
signalling pathways. Gene 778: 145460 (Link) Applications
Injured skeletal muscle model
Methods
Compression loading, mechanical stress application, Western blotting, rolling manipulation, recovery of cells from gels by dextranase treatment and subsequent protein extraction
Cells
HSkMCs (primary human skeletal muscle cells)
Products used
3-D Life Dextran-PEG Hydrogel FG
3-D Life Dextranase
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