3-D Life Hydrogel was used in a microphysiological system (MPS) to build the stromal compartment of a human choroidal in vitro model (Choroid-on-Chip). The model allowed for a controlled immune cell recruitment into the melanocyte-bearing stromal compartment through a vascular monolayer. This choroid-on-chip model was shown to effectively work in efficacy testing of immunosuppressive compounds as well as safety profiling of immunoactivating antibodies.Publications
Cipriano et al. (2022) Human immunocompetent choroid-on-chip: a novel tool for studying ocular effects of biological drugs. Communication Biology 5: 52 (Article) Applications
Safety sceening, drug screening, Organ-on-Chip, Microphysiological System (MPS), perfusion, Choroid-on-Chip
Methods
Injection of 3-D Life Hydrogel into microchannels, fluorescence microscopy, immunofluorescence staining, confocal fluorescence microscopy, microphysiological system, perfusion, KI-67 staining, nuclear (DAPI) staining, hydrogel in a microfluidic platform, Live/Dead staining (FDA/PI Assay)
Cells
PBMCs (human peripheral blood mononuclear cells), T cells, Human melanocytes
Products used
3-D Life RGD Peptide
3-D Life Dextran-CD Hydrogel SG
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