Spheroids of melanoma cells expressing GFP (green fluorescent protein) were embedded in 3-D Life Hydrogel. The effect of drugs on tumor growth and cell death of individual spheroids was followed over 8 days by confocal fluorescence microscopy. Publications
Del Mistro et al. (2022) Focal adhesion kinase plays a dual role in TRAIL resistance and metastatic outgrowth of malignant melanoma. Cell Death and Disease 13:54 (Article) Applications
Drug testing, spheroid culture
Methods
Confocal fluorescence microscopy, Live/Dead staining (Calcein AM/PI)
Cells
WM1346 (human melanoma)
WM1366 (human melanoma)
Malme3M (human melanoma)
A375 (human melanoma)
Products used
3-D Life Dextran-CD Hydrogel SG
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