Mouse pancreas organoids were cultivated in 3-D Life Hydrogel crosslinked with hyaluronic acid or PEG-Link. A 3-D Life Hydrogel composition was found that showed similar mechanical characteristics (stiffness and elasticity) compared to Matrigel®. Different microscopic analyses including Raman microscopy revealed similar growth characteristics and expression patterns of specific stem cell markers between organoids grown in 3-D Life Hydrogel and Matrigel®. This confirmed the suitability of 3-D Life Hydrogel as a potential synthetic alternative to Matrigel® in organoid culture.Publications
Jung et al. (2021) Non-invasive analysis of pancreas organoids in synthetic hydrogels defines material-cell interactions and luminal composition. Biomaterials Science 9(16):5415-5426 (Article) Applications
Organoid culture, cell-matrix interactions
Methods
Confocal fluorescence microscopy, Raman microscopy, pressure force measurement, dark-field microscopy, time-lapse phase-contrast
microscopy
Cells
Pancreatic organoids (murine)
Products used
3-D Life PEG-Link
3-D Life HyLink
3-D Life SG-PVA
3-D Life RGD Peptide
3-D Life PVA-HA Hydrogel
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