Using two-photon microscopy the authors developed a method to tether proteins or peptides in the hydrogel network with micrometer precision. This allows to follow the interaction and effects of immobilised proteins or peptides with cells in subcellular dimensions.Publications
Krüger et al. (2022) Sensitizer-enhanced two-photon patterning of biomolecules in photoinstructive hydrogels. Communications materials 3 (9) (Article) Applications
4D hydrogel, photoresponsive hydrogels, photoinstructive hydrogels
Methods
Laser lithography, one- and two-photon microscopy, TPA-trisNTA photochemistry
Cells
HeLa Kyoto (adenocarcinoma, human)
Products used
3-D Life PVA-PEG Hydrogel SG
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