Detailed protocol on the generation of microspheres for single cell encapsulation.Publications
Sun et al. (2021) CloneSeq - Single-cell clonal 3D culture and analysis protocol. STAR Protocols 2, 100794, December 17, 2021 (Article) Applications
Single cell encapsulation
Methods
Microsphere production, microsphere cultivation, fluorescent labeling of hydrogel via biotin-streptavidin attachment, bead production
Cells
PC9 (lung adenocarcinoma, human), ZHBTc4 ESCs (mouse embryonic stem cells)
Products used
3-D Life PEG-Link
3-D Life CD-Link
3-D Life FG-Dextran
3-D Life 10x CB Buffer (pH 7.2)
3-D Life Dextranase
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