This book chapter describes in detail the 3-D Life Hydrogel platform technology as well as selected applications of in vitro cultures. Publications
Angres, B. and Wurst, H. (2017) 3-D Life biomimetic hydrogels: A modular system for cell environment design. Przyborski, S. (ed.) Technology Platforms for 3D Cell Culture, A User`s Guide. pp. 197-221. (Link) Applications
Tumor-stroma model
Methods
Fluorescence microscopy, Live/Dead staining (Calcein AM/PI), Sytox Green Assay (nuclear staining), phalloidin staining, formaldehyde fixation
Cells
MCF-7 (mamma carcinoma, human), Human dermal fibroblasts
Products used
3-D Life RGD Peptide
3-D Life Thioglycerol
3-D Life Dextran-CD Hydrogel FG
3-D Life Dextranase
3-D Life PVA-PEG Hydrogel FG
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