The 3-D Life Hydrogel technology was used to prepare maleimide-modified human or sheep serum albumin which was crosslinked with PEG-Link and supplemented with hyaluronan. Chondrocytes cultured in these gels were vital and kept their differentiation. Moreover, the hydrogel proved to be non-permissive for endothelial cells to enter the gel in CAM assays as well as after implantation in NOD/SCID mice. Thus, the hydrogel was shown to be a good candidate to suppress pathological blood vessel formation when used for chondrocyte encapsulation for autologous chondrocyte implantation.Publications
Scholz, B., et al. (2010) Suppression of Adverse Angiogenesis in an Albumin-based Hydrogel for Articular Cartilage and Intervertebral Disc Regeneration. Eur. Cell. Mater. 20:24-37 (download) Applications
Implantation, angiogenesis, cell invasion, chondrogenesis, cartilage research, biocompatibility studies, tissue regeneration
Methods
Culture in multiwell inserts, invasion assay, transwell migration assay, implantation in NOD SCID mice, chorioallantoic membrane (CAM) assay, recovery of cells from serum albumin/PEG hydrogel by digestion with proteinase K and subsequent qRT-PCR, hydrogel-tissue freezing and cryostat sectioning
Cells
Chondrocytes, HUVECs (Human Umbilical Vein Endothelial Cells), Invertebral disc chondrocytes, Human chondrocytes
Products used
maleimide modified human and sheep serum albumin
3-D Life PEG-Link
Please send a message to order products or if you would like to know more about Cellendes:
Cellendes GmbH
Gerhard-Kindler-Str. 8
D-72770 Reutlingen
Germany
Ph.: +49 7121 15940 0
Fax: +49 7121 15940 99
info@cellendes.com